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MIBE Seminar: Responsive protein nanostructures as reporters for cell signaling in Correlative Light and Electron Microscopy (CLEM)

Topic: Responsive protein nanostructures as reporters for cell signaling in Correlative Light and Electron Microscopy (CLEM)

Speaker: Filipa Goncalves (Westmeyer Group)

Date and Time: Tuesday 28 May 2024, 1:00 pm

Abstract: Correlative Light and Electron Microscopy (CLEM) is a technique that enables the visualization of biological events, both functionally and structurally, using fluorescence microscopy (FM) and electron microscopy (EM), respectively. The fundamental challenge lies in the paucity of reporters capable of reliably detecting changes in a cell across both microscopy modalities, thus preventing a comprehensive understanding of biological systems and their interactions. To address this limitation, we introduced EMcapsulins multiplexed gene reporters for CLEM. This approach involves the incorporation of fluorescent tags, metal-binding domains, calcium indicators, and other molecular moieties capable of detecting cellular events. Recent research in our group has demonstrated the readability of these nanostructures in EM, presenting distinguishable classes in mammalian cells, Drosophila, and mouse brains. Furthermore, these structures efficiently label cellular components in both FM and EM. One of our current foci is to engineer these nanostructures to respond effectively to biological stimuli, enabling the translation of cellular states with high sensitivity and temporal resolution in both FM and EM.

More information

The MIBE Seminar will take place in our lecture hall E.126 (Boltzmannstr. 11, 85748 Garching) and will be live streamed via zoom.

The zoom details for all online seminar talks this semester are:

Meeting Name: MIBE Seminar Talks, Tuesdays 1:00 pm
Meeting-ID: 698 5129 8624
Password: mibe

The MIBE Seminar counts as subject specific qualification for doctoral candidates.
Registration here.

Further Program SUMMER TERM 2024

Jun 11, 2024    Florian Schaff             Pfeiffer Group             Title tbd

Jun 18, 2024    Henriette Bast             Pfeiffer Group             Clinical Dark-Field Radiography of Human Lungs

Jun 25, 2024    Dominik John              Herzen Group             From Images to Insights: Quantitative Phase-                                                                                                Contrast Imaging for the Analysis of Biomedical
                                                                                                Specimens and Materials at a Synchrotron Source

Jul 09, 2024     Francesca Gasparin   Ntziachristos Group     Optoacoustic Microscopy
                         & Ludwig Englert